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Journal: Frontiers in Pharmacology
Article Title: Huangkui capsule combined with finerenone attenuates diabetic nephropathy by regulating the JAK2/STAT3 signaling pathway based on network pharmacology, molecular docking, and experimental verification
doi: 10.3389/fphar.2025.1625286
Figure Lengend Snippet: IHC analysis of tubular injury markers (n = 3 per group, scale bar 50/100 μm, ‾X ± SD), which was analyzed by one-way ANOVA. KIM-1/NGAL expression: the CDI group showed decreased KIM-1 (45%) and NGAL (52%) vs. model (***p < 0.001) and vs. monotherapy (*p < 0.05) groups.
Article Snippet: For IHC staining, sections were stained with antibodies to neutrophil gelatinase-associated lipocalin (NGAL) (1:200) (BosterBio, Wuhan, China, No. 83221-2-RR) and
Techniques: Expressing
Journal: Frontiers in Pharmacology
Article Title: Huangkui capsule combined with finerenone attenuates diabetic nephropathy by regulating the JAK2/STAT3 signaling pathway based on network pharmacology, molecular docking, and experimental verification
doi: 10.3389/fphar.2025.1625286
Figure Lengend Snippet: Western blotting analysis of the inhibition of inflammation, renal tubular injury markers, and STAT3 activation by the combination treatment before transfection in HK-2 cells (n = 3 per group, ‾X ± SD), which was analyzed by one-way ANOVA. The CDI group showed downregulated p-STAT3, IL-6, TNF-α, KIM-1, and NGAL vs. LPS + high glucose (***p < 0.001) and vs. monotherapy (*p < 0.05) groups.
Article Snippet: For IHC staining, sections were stained with antibodies to neutrophil gelatinase-associated lipocalin (NGAL) (1:200) (BosterBio, Wuhan, China, No. 83221-2-RR) and
Techniques: Western Blot, Inhibition, Activation Assay, Transfection